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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1988-6-6
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pubmed:abstractText |
The inhibitory effects of Ca2+-binding proteins on tyrosine phosphorylation of p36 protein isolated from bovine intestinal epithelium by immunoprecipitated p130fps were investigated. S-100 protein dose dependently inhibited the p36 phosphorylation, and calmodulin weakly depressed the phosphorylation, whereas parvalbumin and troponin C had no significant effects. The S-100 preparation purified from bovine brain did not contain phosphatase activity or ATPase activity. The concentration of ATP did not affect the S-100-mediated inhibition of phosphorylation but the substrate protein, p36, reversed the inhibition. S-100 similarly inhibited the tyrosine phosphorylation of p36 by p60src but did not affect the p36 phosphorylation by protein kinase C. S-100 inhibited the tyrosine kinase activity of p130fps using the other substrates tested as well. These results suggest that S-100 interacts with the substrate binding site of retroviral tyrosine-specific protein kinases and may play a regulatory role in the tyrosine phosphorylation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6438-41
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2834378-Adenosine Triphosphatases,
pubmed-meshheading:2834378-Animals,
pubmed-meshheading:2834378-Avian Sarcoma Viruses,
pubmed-meshheading:2834378-Calcium-Binding Proteins,
pubmed-meshheading:2834378-Calmodulin,
pubmed-meshheading:2834378-Cattle,
pubmed-meshheading:2834378-Chickens,
pubmed-meshheading:2834378-Dose-Response Relationship, Drug,
pubmed-meshheading:2834378-Molecular Weight,
pubmed-meshheading:2834378-Phosphorylation,
pubmed-meshheading:2834378-Protein Kinase C,
pubmed-meshheading:2834378-Protein-Tyrosine Kinases,
pubmed-meshheading:2834378-Rabbits,
pubmed-meshheading:2834378-S100 Proteins,
pubmed-meshheading:2834378-Tyrosine,
pubmed-meshheading:2834378-Viral Proteins
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pubmed:year |
1988
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pubmed:articleTitle |
Modulation of tyrosine phosphorylation of p36 and other substrates by the S-100 protein.
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pubmed:affiliation |
Department of Molecular and Cellular Pharmacology, Mie University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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