Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-5-3
pubmed:abstractText
To investigate the relationship between inositol lipid hydrolysis and reactive oxygen-intermediate (ROI) production in macrophages we have examined the effect of platelet-activating factor (PAF) on normal bone marrow-derived macrophages. Addition of PAF to macrophages prelabelled with [3H]inositol caused a marked and rapid increase in [3H]inositol trisphosphate levels. Similarly when PAF was added to [3H]-glycerol prelabelled macrophages there was a rapid increase in 1,2-diacyl[3H]glycerol levels. These events preceded any increase in the rate of PAF-stimulated ROI production by a discernible period of several seconds. Increasing concentrations of PAF led to a markedly similar increase in both ROI production and [3H]inositol lipid hydrolysis suggesting that inositol lipid hydrolysis may lead to the generation of ROI in macrophages. Further evidence that this is the case came from experiments in which pretreatment of macrophages with phorbol esters was shown to inhibit both PAF-stimulated [3H]inositol phosphate production and ROI production to a markedly similar degree. Similarly pertussis toxin inhibited both PAF-stimulated ROI production and [3H]inositol phosphate production. Phorbol esters were shown to activate ROI production in normal bone marrow-derived macrophages whereas the Ca2+ ionophore, A23187, did not. These experiments suggest that PAF stimulates a pertussis toxin-sensitive activation of inositol lipid hydrolysis leading to the formation of inositol trisphosphate and diacylglycerol. The diacylglycerol formed can then activate protein kinase C leading to the stimulation of ROI production in normal bone marrow-derived macrophages.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-13428781, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-2412225, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-2432882, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-2986126, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-2987206, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-2987951, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-2993272, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-3006836, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-3007118, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-3026354, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-3493133, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-3545319, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-3922980, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-4118412, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6089766, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6094010, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6100475, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6142883, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6144681, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6232463, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6302012, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6303343, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6309563, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6309875, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6311626, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6313047, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6317241, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6333869, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6339357, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6386073, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-6736651, http://linkedlifedata.com/resource/pubmed/commentcorrection/2833227-7149240
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1,2-diacylglycerol, http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin, http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides, http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates, http://linkedlifedata.com/resource/pubmed/chemical/Oxygen, http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 4,5-Diphosphate, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols, http://linkedlifedata.com/resource/pubmed/chemical/Platelet Activating Factor, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate, http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
249
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
839-45
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Platelet-activating factor-induced hydrolysis of phosphatidylinositol 4,5-bisphosphate stimulates the production of reactive oxygen intermediates in macrophages.
pubmed:affiliation
Department of Biochemistry & Applied Molecular Biology, University of Manchester Institute of Science and Technology, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't