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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-3-23
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pubmed:abstractText |
Human interleukin-1 beta (IL-1 beta) has two cysteines located at amino acid residues 8 and 71 of the mature protein consisting 153 amino acids. To clarify the role of these characteristic cysteine residues in IL-1 beta, at first, an expression plasmid for site-specific mutagenesis has been constructed by inserting the ori and intergenic region of phage f1 into the IL-1 beta expression vector. The plasmid can be used not only for isolation of the modified IL-1 beta gene but for expression of the mutant protein in Escherichia coli. Using this plasmid, each of the cysteine codons in IL-1 beta gene was changed to serine or alanine codon, or deleted. The modified IL-1 beta showed that the two cysteine residues in IL-1 beta are not essential for biological activity but not to be eliminated for the maintenance of the functional structure of IL-1 beta.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
150
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1106-14
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2829892-Codon,
pubmed-meshheading:2829892-Cysteine,
pubmed-meshheading:2829892-DNA, Recombinant,
pubmed-meshheading:2829892-DNA, Single-Stranded,
pubmed-meshheading:2829892-DNA Restriction Enzymes,
pubmed-meshheading:2829892-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2829892-Escherichia coli,
pubmed-meshheading:2829892-Humans,
pubmed-meshheading:2829892-Interleukin-1,
pubmed-meshheading:2829892-Melanoma,
pubmed-meshheading:2829892-Molecular Weight,
pubmed-meshheading:2829892-Mutation,
pubmed-meshheading:2829892-Plasmids,
pubmed-meshheading:2829892-Structure-Activity Relationship,
pubmed-meshheading:2829892-Tumor Cells, Cultured
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pubmed:year |
1988
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pubmed:articleTitle |
Site-specific mutagenesis of the human interleukin-1 beta gene: structure-function analysis of the cysteine residues.
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pubmed:affiliation |
Laboratories of Cellular Technology, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
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pubmed:publicationType |
Journal Article
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