Linked Life Data

2829427

Source:http://linkedlifedata.com/resource/pubmed/id/2829427

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-3-11
pubmed:databankReference
pubmed:abstractText
A 275-bp sequence in the short unique region of human cytomegalovirus (HCMV) strain AD169 contains a series of five imperfect 18-bp repeats with homology to the SV40 and HCMV major immediate early enhancers. Plasmids containing these putative HCMV enhancer sequences linked to the human beta-globin gene were transiently transfected into HeLa cells, and the ability of the HCMV sequences to activate beta-globin transcription was assayed. The repeat-containing region stimulated transcription in a position- and orientation-independent manner, to approximately the same degree as that of the SV40 enhancer. A promoter located immediately 3' to the enhancer in the HCMV genome is active only in the presence of the enhancer sequences. Transcription from this HCMV promoter was analysed by a combination of S1 nuclease protection mapping and Northern blotting. At immediate early times postinfection, at least four extremely abundant differentially spliced mRNAs were detected; these RNAs constitute a previously unknown class of immediate early transcript.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
406-16
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
An enhancer element in the short unique region of human cytomegalovirus regulates the production of a group of abundant immediate early transcripts.
pubmed:affiliation
M.R.C. Laboratory of Molecular Biology, Cambridge, England.
pubmed:publicationType
Journal Article