Linked Life Data

2824544

Source:http://linkedlifedata.com/resource/pubmed/id/2824544

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1987-12-29
pubmed:abstractText
Adsorption isotherms of proteins were measured by frontal chromatography in a miniaturized chromatographic system with short, narrow-bore columns. The adsorbents were microparticulate, macroporous siliceous cation exchangers, used in the high-performance liquid chromatography of proteins, and the range of mobile phase compositions was similar to that used in elution chromatography for their separation. Adsorption of protein on the wetted stainless-steel parts of the system was reduced by coating the metal surface with a hydrophilic polymer layer. The adsorption isotherms of proteins were concave down and, in most cases, well represented by the Langmuir model. However, the data could also be fitted with reasonable accuracy to the isotherm equation by Jovanovi?. Although the siliceous cation exchangers were of different provenance, the isotherms of the proteins were similar. Isotherms measured by the shake-flask method were found to be identical with those obtained by frontal chromatography.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
406
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-94
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Adsorption isotherms on high-performance liquid chromatographic sorbents. II. Proteins on cation exchangers with silica support.
pubmed:affiliation
Department of Chemical Engineering, Yale University, New Haven, CT 06520.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.