|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1987-11-25
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Genomic DNA hybridizations reveal that the mini-exon gene repeats of Trypanosoma (Nannomonas) congolense contain from one to greater than 20 internal tandem repeats of 190 base pairs (bp). The sequences of two mini-exon gene repeats were determined, one with three internal 190 bp repeats and one with a single 190 bp sequence. These internal repeats appear just after a very dC/dT-rich region, in the non-transcribed spacer regions between adjacent mini-exon transcription units. They do not occur in the genomes of the three other Trypanosoma species nor in the mini-exon gene repeats of four other kinetoplastids previously investigated. The mini-exon coding region of T. congolense differs in four positions from the T. brucei mini-exon sequence and in one position from a consensus sequence of the mini-exons found in other trypanosomatids. Pulse field gradient gels show that the mini-exon gene repeats of T. congolense are on large, greater than 2 megabase pair, chromosome(s).
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0166-6851
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
25
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
113-22
|
|
pubmed:dateRevised |
2008-8-19
|
|
pubmed:meshHeading |
pubmed-meshheading:2823133-Animals,
pubmed-meshheading:2823133-Autoradiography,
pubmed-meshheading:2823133-Base Sequence,
pubmed-meshheading:2823133-Cloning, Molecular,
pubmed-meshheading:2823133-DNA,
pubmed-meshheading:2823133-DNA Restriction Enzymes,
pubmed-meshheading:2823133-Deoxyribonuclease HindIII,
pubmed-meshheading:2823133-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:2823133-Electrophoresis, Agar Gel,
pubmed-meshheading:2823133-Exons,
pubmed-meshheading:2823133-Genes,
pubmed-meshheading:2823133-Molecular Sequence Data,
pubmed-meshheading:2823133-Nucleic Acid Hybridization,
pubmed-meshheading:2823133-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2823133-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2823133-Trypanosoma congolense
|
|
pubmed:year |
1987
|
|
pubmed:articleTitle |
Mini-exon gene repeats of Trypanosoma (Nannomonas) congolense have internal repeats of 190 base pairs.
|
|
pubmed:affiliation |
Department of Biochemistry, University of Iowa, Iowa City 52242-1193.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
