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rdf:type |
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lifeskim:mentions |
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0033413,
umls-concept:C0036667,
umls-concept:C0043292,
umls-concept:C0205177,
umls-concept:C0205254,
umls-concept:C0312418,
umls-concept:C0443199,
umls-concept:C0597094,
umls-concept:C1840025
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pubmed:issue |
8
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pubmed:dateCreated |
1987-12-4
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pubmed:abstractText |
We investigated the conformation of the X-linked mouse hypoxanthine-guanine phosphoribosyltransferase gene (HPRT) promoter region both in chromatin from the active and inactive X chromosomes with DNase I and in naked supercoiled DNA with S1 nuclease. A direct comparison of the chromatin structures of the active and inactive mouse HPRT promoter regions was performed by simultaneous DNase I treatment of the active and inactive X chromosomes in the nucleus of interspecies hybrid cells from Mus musculus and Mus caroli. Using a restriction fragment length polymorphism to distinguish between the active and inactive HPRT promoters, we found a small but very distinct difference in the DNase I sensitivity of active versus inactive chromatin. We also observed a single DNase I-hypersensitive site in the immediate area of the promoter which was present only on the active X chromosome. Analysis of the promoter region by S1 nuclease digestion of supercoiled plasmid DNA showed an S1-sensitive site which maps adjacent to or within the DNase I-hypersensitive site found in chromatin but upstream of the region minimally required for normal HPRT gene expression.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-14428474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-2423876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-2428611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-2982820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-2984578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3011277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3022138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3023844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3455894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3456175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3839456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3909940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3909948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-3927483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6087154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6088070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6099325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6101195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6222378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6287292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6288265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6308469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6319416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6322123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6326107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6327689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6364959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6774262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6871996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6956879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-6959140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-943046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823112-948749
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2994-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2823112-Animals,
pubmed-meshheading:2823112-Chromatin,
pubmed-meshheading:2823112-DNA, Superhelical,
pubmed-meshheading:2823112-Deoxyribonuclease I,
pubmed-meshheading:2823112-Endonucleases,
pubmed-meshheading:2823112-Genes,
pubmed-meshheading:2823112-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:2823112-Liver,
pubmed-meshheading:2823112-Male,
pubmed-meshheading:2823112-Mice,
pubmed-meshheading:2823112-Promoter Regions, Genetic,
pubmed-meshheading:2823112-Single-Strand Specific DNA and RNA Endonucleases,
pubmed-meshheading:2823112-X Chromosome
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pubmed:year |
1987
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pubmed:articleTitle |
Nuclease sensitivity of the mouse HPRT gene promoter region: differential sensitivity on the active and inactive X chromosomes.
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pubmed:affiliation |
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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