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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1987-11-2
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pubmed:abstractText |
An early antigen of human cytomegalovirus (HCMV) was purified from infected cells as a 72K polypeptide by immunoaffinity chromatography using a monoclonal antibody. It was located in both nucleus and cytoplasm, and was non-glycosylated and undetectable on the surface of infected cells. Known seropositive subjects had antibody against the purified protein and it elicited proliferative T cell responses in 10 of 16 subjects. Five of 14 T cell lines established in response to the purified protein were predominantly CD8+ and of these two showed major histocompatibility complex-restricted cytotoxicity against HCMV-infected cells. This provides further evidence that antigens expressed at early times may be targets for the immune response during persistent HCMV infection.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68 ( Pt 9)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2371-8
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pubmed:dateRevised |
2005-5-13
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pubmed:meshHeading |
pubmed-meshheading:2821176-Antibody Formation,
pubmed-meshheading:2821176-Antigen-Antibody Complex,
pubmed-meshheading:2821176-Antigens, Surface,
pubmed-meshheading:2821176-Antigens, Viral,
pubmed-meshheading:2821176-Chromatography, Affinity,
pubmed-meshheading:2821176-Cytomegalovirus,
pubmed-meshheading:2821176-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2821176-Fibroblasts,
pubmed-meshheading:2821176-Humans,
pubmed-meshheading:2821176-Immediate-Early Proteins,
pubmed-meshheading:2821176-Immunity, Cellular,
pubmed-meshheading:2821176-Molecular Weight
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pubmed:year |
1987
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pubmed:articleTitle |
Immunoaffinity purification of a 72K early antigen of human cytomegalovirus: analysis of humoral and cell-mediated immunity to the purified polypeptide.
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pubmed:affiliation |
Department of Medicine, Royal Postgraduate Medical School, London, U.K.
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pubmed:publicationType |
Journal Article
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