Linked Life Data

2808400

Source:http://linkedlifedata.com/resource/pubmed/id/2808400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
32
pubmed:dateCreated
1989-12-15
pubmed:abstractText
In vitro transcription elongation, in HeLa whole cell extract, is partially blocked at the Ad2 and synthetic (8 Ts) attenuation sites when transcription elongation is performed at increasing KCl concentrations (0.2-0.5 M KCl) but not at the standard KCl concentration (50 mM). Furthermore, if briefly initiated transcription complexes are chromatographed on Sephacryl column at increased KCl concentration and the complexes, which are eluted in the void volume, are diluted to 50 mM KCl, they will elongate a substantial fraction of the briefly initiated nascent RNA into attenuated RNA. The addition of a crude preparation of a stimulatory factor to the complexes diluted to 50 mM KCl prevents the elongation blocks. Based on the notion that putative elongation factors dissociate from the transcription complexes at increasing KCl concentrations and in the Sephacryl column they elute after the initiation complexes, we suggest that the column-purified transcription complexes, which have been depleted of elongation factors, can serve as a model system for determining the activities of factors during elongation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18926-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Transcription complexes synthesizing attenuated RNA can serve as a model system for analyzing elongation factors.
pubmed:affiliation
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't