Linked Life Data

2792998

Source:http://linkedlifedata.com/resource/pubmed/id/2792998

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-11-21
pubmed:abstractText
Electrolyte and fluid transport across the corneal epithelium plays an important role for the maintenance of corneal transparency. Little is known about the cellular mechanisms underlying the transepithelial transport phenomena. We have therfore started to investigate the transmembranal transport processes in cultured corneal epithelial cells. Studies were performed in an established corneal epithelial cell line from the rabbit (SIRC cells) and in primary cultured bovine corneal epithelial cells. In these cells, intracellular pH was continuously measured using the pH-sensitive absorbance of intracellularly trapped dimethylcarboxyfluorescein. In both cell types, SIRC cells and cultured bovine corneal epithelial cells, evidence was found in favor of a sodium-dependent and amiloride-sensitive Na/H exchange. In cultured bovine corneal epithelial cells, Na/H exchange was shown to play an important role in intracellular pH recovery after an acid load. Furthermore, as has been demonstrated in other epithelia, Na+/H+ exchange may also be important for transepithelial corneal transport.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0723-8045
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
[Measuring intracellular pH in cultivated corneal epithelial cells].
pubmed:publicationType
Journal Article, English Abstract