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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1989-7-14
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pubmed:abstractText |
We report a new methodology for the long-term growth of malignant T-lymphoblasts from patients with T-cell acute lymphoblastic leukemia (T-ALL) and T-cell lymphoblastic lymphoma (T-LL). When malignant cells were cultured in the presence of insulin-like growth factor I under hypoxic conditions, cellular proliferation occurred that resulted in the establishment of immortal cell lines from ten of 12 patient tumors. Authenticity of each cell line was verified by a direct comparison of the immunophenotype, karyotype, and immunogenotype with the patient's tumor cells. This improved method of cell culture permits frequent establishment of cell lines from patients with T-ALL/T-LL, thereby aiding in analysis of thymocyte transformation and neoplasia.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2182-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2786436-Adolescent,
pubmed-meshheading:2786436-Adult,
pubmed-meshheading:2786436-Aged,
pubmed-meshheading:2786436-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2786436-Blotting, Southern,
pubmed-meshheading:2786436-Cell Division,
pubmed-meshheading:2786436-Cell Line,
pubmed-meshheading:2786436-Child,
pubmed-meshheading:2786436-Child, Preschool,
pubmed-meshheading:2786436-DNA Nucleotidylexotransferase,
pubmed-meshheading:2786436-Gene Rearrangement,
pubmed-meshheading:2786436-Humans,
pubmed-meshheading:2786436-Insulin-Like Growth Factor I,
pubmed-meshheading:2786436-Karyotyping,
pubmed-meshheading:2786436-Leukemia-Lymphoma, Adult T-Cell,
pubmed-meshheading:2786436-Male,
pubmed-meshheading:2786436-Phenotype,
pubmed-meshheading:2786436-T-Lymphocytes,
pubmed-meshheading:2786436-Tumor Cells, Cultured
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pubmed:year |
1989
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pubmed:articleTitle |
Long-term growth of malignant thymocytes in vitro.
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pubmed:affiliation |
Department of Pediatrics, Stanford University Medical School, Palo Alto, CA 94304-2099.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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