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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-6-29
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pubmed:abstractText |
The epidermal growth factor (EGF)-receptor is composed of an extracellular ligand-binding region connected by a single transmembrane region to the cytoplasmic kinase domain. In spite of its importance for understanding signal transduction, the ligand-binding domain of the EGF-receptor is not yet defined. We describe the identification of a major ligand-binding domain of the EGF-receptor by utilizing chimeras between the human EGF-receptor and the chicken EGF-receptor. This approach is based on the fact that murine EGF binds to the chicken EGF-receptor with 100-fold lower affinity as compared to the human EGF-receptor. Hence, the substitution of various domains of the chicken EGF-receptor by domains of the human EGF-receptor may restore the higher binding affinity towards EGF, characteristic of the human receptor. We show that chimeric chicken/human EGF-receptor, which contains domain III of the extracellular region of the human receptor, behaves like the human EGF-receptor with respect to EGF binding affinity and biological responsiveness. However, a chimeric chicken/human EGF-receptor containing domains I and II of the human receptor behaves like the chicken rather than the human EGF-receptor. Moreover, two different monoclonal antibodies which compete for the binding of EGF to EGF-receptor recognize specifically domain III of the human EGF-receptor. It is concluded that domain III which is flanked by the two cysteine-rich domains is a major ligand-binding domain of the EGF-receptor.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-161245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-161246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-222468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-2431286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-2824188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-2982501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-2992362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3017977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3023396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3260004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3260329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3260862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3291942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3291943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3498122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3499230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-3501826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-382984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-6157683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2785915-6328312
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
421-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2785915-Animals,
pubmed-meshheading:2785915-Antibodies, Monoclonal,
pubmed-meshheading:2785915-Binding, Competitive,
pubmed-meshheading:2785915-Binding Sites,
pubmed-meshheading:2785915-Chickens,
pubmed-meshheading:2785915-Chimera,
pubmed-meshheading:2785915-Epidermal Growth Factor,
pubmed-meshheading:2785915-Humans,
pubmed-meshheading:2785915-Immunochemistry,
pubmed-meshheading:2785915-Kinetics,
pubmed-meshheading:2785915-Receptor, Epidermal Growth Factor
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pubmed:year |
1989
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pubmed:articleTitle |
Functional analysis of the ligand binding site of EGF-receptor utilizing chimeric chicken/human receptor molecules.
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pubmed:affiliation |
Rorer Biotechnology, Inc., King of Prussia, PA 19406.
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pubmed:publicationType |
Journal Article
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