Linked Life Data

2785255

Source:http://linkedlifedata.com/resource/pubmed/id/2785255

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-6-2
pubmed:abstractText
Two classes of ligand-binding molecules, (1) monoclonal antibodies (MABs) and (2) lectins, were used as probes to investigate the cell biology of human trabecular cells. We raised MABs by using human trabecular meshwork as the immunogen. One MAB (KAa25D7) consistently recognized two polypeptides with molecular weights of approximately 42 and 49 kilodaltons. The former comigrated with actin and the latter was shown by fractionation and extraction studies to be a macromolecular complex. Four lectins (wheat germ agglutinin, concanavalin A, Phaseolus vulgaris leukoagglutinin I, and Ulex europaeus agglutinin I) were used to identify the glycosylated polypeptides in human trabecular meshwork on Western blots. Compared to normal age-matched controls, there was a significant decrease in binding of the lectins to most polypeptides of the trabecular meshwork samples that were obtained from 4 eyes of 2 patients with primary open-angle glaucoma. Our preliminary studies show that lectins applied to Western blots and MABs are useful methods to probe the cell biology of human trabecular meshwork in health and disease.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0030-3747
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Monoclonal antibodies and lectins as probes for investigation of the cell biology of human trabecular meshwork: a preliminary report.
pubmed:affiliation
Department of Ophthalmology, University of Chicago, Ill.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't