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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-9-1
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pubmed:abstractText |
The apparent steady-state fluorescence anisotropy of DPH- or TMA-DPH-labeled washed rat platelets is strongly affected by factors that also influence the turbidity by these platelet suspensions. Sonicated preparations from platelet lipids have a low turbidity and give anisotropy values which are hardly affected by the experimental conditions. We studied the effect of four high-fat diets on membrane fluidity, lipid composition and activation tendency of washed platelets. The diets contained 50 energy% of oils with different levels of saturated and (poly)unsaturated fatty acids. Only small diet-induced differences in DPH fluorescence anisotropy were found, which were comparable for intact platelets and platelet lipids. These differences were unrelated to the degree of saturation of the dietary fatty acids. Platelets from rats fed mainly saturated fatty acids differed significantly from other diet groups in a higher unsaturation degree of phospholipids and a lower cholesterol/phospholipid ratio, but this was not detected by DPH in terms of decreased anisotropy. These platelets aggregated less than other platelets in response to thrombin or collagen. The lower response to collagen persisted in indomethacin-treated platelets activated with the thromboxane A2 mimetic U46619, indicating a different sensitivity of these platelets for thromboxane A2. We conclude that in rat platelets: (a) the overall membrane fluidity and phospholipid unsaturation degree are subject to strong homeostatic control; (b) steady-state anisotropy with DPH or TMA-DPH label is inadequate to reveal subtile changes in lipid profile; (c) changes in platelet responsiveness to thrombin and thromboxane A2, rather than (plasma) membrane fluidity, determine the effect of dietary fatty acids on platelet aggregation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(4-(trimethylamino)phenyl)-6-pheny...,
http://linkedlifedata.com/resource/pubmed/chemical/15-Hydroxy-11 alpha,9...,
http://linkedlifedata.com/resource/pubmed/chemical/Dietary Fats,
http://linkedlifedata.com/resource/pubmed/chemical/Diphenylhexatriene,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Fish Oils,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Oils,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Aggregation Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin Endoperoxides...,
http://linkedlifedata.com/resource/pubmed/chemical/Thromboxane A2,
http://linkedlifedata.com/resource/pubmed/chemical/Thromboxane B2,
http://linkedlifedata.com/resource/pubmed/chemical/coconut oil,
http://linkedlifedata.com/resource/pubmed/chemical/palm oil,
http://linkedlifedata.com/resource/pubmed/chemical/sunflower seed oil
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
1004
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
252-60
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2752022-15-Hydroxy-11 alpha,9...,
pubmed-meshheading:2752022-Animals,
pubmed-meshheading:2752022-Blood Platelets,
pubmed-meshheading:2752022-Dietary Fats,
pubmed-meshheading:2752022-Diphenylhexatriene,
pubmed-meshheading:2752022-Fatty Acids,
pubmed-meshheading:2752022-Fish Oils,
pubmed-meshheading:2752022-Fluorescence Polarization,
pubmed-meshheading:2752022-Fluorescent Dyes,
pubmed-meshheading:2752022-Indomethacin,
pubmed-meshheading:2752022-Male,
pubmed-meshheading:2752022-Membrane Fluidity,
pubmed-meshheading:2752022-Plant Oils,
pubmed-meshheading:2752022-Platelet Aggregation,
pubmed-meshheading:2752022-Platelet Aggregation Inhibitors,
pubmed-meshheading:2752022-Prostaglandin Endoperoxides, Synthetic,
pubmed-meshheading:2752022-Rats,
pubmed-meshheading:2752022-Rats, Inbred Strains,
pubmed-meshheading:2752022-Thromboxane A2,
pubmed-meshheading:2752022-Thromboxane B2
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pubmed:year |
1989
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pubmed:articleTitle |
Influence of dietary fatty acids on membrane fluidity and activation of rat platelets.
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pubmed:affiliation |
Department of Biochemistry, University of Limburg, Maastricht, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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