Linked Life Data

2732287

Source:http://linkedlifedata.com/resource/pubmed/id/2732287

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1989-7-25
pubmed:abstractText
Secondary equilibria in reversed-phase liquid chromatography have been investigated as a means of enhancing selectivity and optimizing separations of nucleic acid constituents. The retention behavior of various nucleotides, nucleosides and modified compounds has been examined as a function of five different metal ion additives in the mobile phase: K+, Mg2+, Mn2+, Ni2+ and Zn2+. Complexation of the solute molecules with the metal ions changes the electronic structure and alters solute-solvent interactions. Alkali and alkaline earth metals bind primarily to phosphate groups while transition metals also interact with the N7 of purine bases. All nucleotides were found to be eluted very close to the void volume of the high-performance liquid chromatographic column without any metal additive, but retention increased as the concentration of a given cation increased. The transition metals were found to have the greatest effect, with affinities for nucleotide monophosphates on the order of 100 times greater than potassium, and 10 times that of magnesium. Differences in affinity based upon phosphate structure (i.e., cyclic vs. linear), phosphate position (e.g., 2'- vs. 3'-monophosphates), and base modification were also noted. The retention of most nucleosides, unlike the charged compounds, remained relatively constant as the ionic strength or type of cation was varied. Also, improvements were obtained in the resolution of some oligonucleotides with the addition of divalent ions to a potassium buffer mobile phase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
468
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-79
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Retention modification of nucleic acid constituents in reversed-phase high-performance liquid chromatography.
pubmed:affiliation
Oak Ridge National Laboratory, Analytical Chemistry Division, TN 37831-6120.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.