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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1989-7-7
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pubmed:abstractText |
Sonication of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-sn-glycero-3-phosphocholine (lysoPC, up to approximately 30 mol %) produces small unilamellar vesicles (SUV, 250-265 A diameter). Phosphorus-31 NMR of the POPC/lysoPC vesicles gives rise to four distinct peaks for POPC and lysoPC in the outer and in the inner bilayer leaflet which can be used to localize and quantify the phospholipids in both vesicle shells. Addition of paramagnetic ions (3 mM Pr3+) enhances outside/inside chemical shift differences and allows monitoring of membrane integrity by the absence of Pr3+ in the vesicle interior. 31P NMR shows that lysoPC in these highly curved POPC/lysoPC vesicles prefers the outer bilayer leaflet. LysoPC incorporation into POPC SUV furthermore causes a substantial and concentration-dependent decrease in spin-spin relaxations (T*2) of the outside POPC phosphorus signals from 55 ms for pure POPC vesicles (v1/2, 5.8 Hz) to 29.5 ms (v1/2, 10.8 Hz) for POPC/lysoPC vesicles containing 25 mol % lysoPC. Our findings are consistent with the idea of a cone-shaped lysoPC molecule which, for geometric reasons, is preferentially accommodated in the outer bilayer leaflet. LysoPC incorporation into POPC SUV restricts POPC headgroup motion and tightens phospholipid packing, but only in the outer bilayer shell.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-14187392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-213099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-272647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-3349040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-3767957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-391283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-4425071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-4475349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-465468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-486446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-5777332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-5875201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-836836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2720071-836837
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
789-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1989
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pubmed:articleTitle |
Lysophosphatidylcholine stabilizes small unilamellar phosphatidylcholine vesicles. Phosphorus-31 NMR evidence for the "wedge" effect.
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pubmed:affiliation |
Hormel Institute, University of Minnesota, Austin 55912.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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