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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1989-6-13
|
| pubmed:abstractText |
Five murine hybridoma clones, producing monoclonal antibodies (MAbs) to bovine interferon-alpha (BoIFN-alpha) were established. One of these, F12, secreted MAbs giving high titers, in ELISA tests, neutralizing both BoIFN-alpha, -alpha C, and BoIFN-alpha D activities, belonging to the mouse IgG1 class, and having a binding affinity constant of 10(8) M-1. F12 MAbs were used for immunoaffinity purification of BoIFN-alpha, and recombinant BoIFN-alpha C from Escherichia coli extracts was purified to homogeneity in SDS-PAGE analysis and to a specific activity of 2 X 10(8) U/mg with 90% recovery of activity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0197-8357
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
189-93
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2715671-Animals,
pubmed-meshheading:2715671-Antibodies, Monoclonal,
pubmed-meshheading:2715671-Chromatography, Affinity,
pubmed-meshheading:2715671-Cross Reactions,
pubmed-meshheading:2715671-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2715671-Hybridomas,
pubmed-meshheading:2715671-Immunoassay,
pubmed-meshheading:2715671-Immunoglobulin G,
pubmed-meshheading:2715671-Interferon Type I,
pubmed-meshheading:2715671-Mice,
pubmed-meshheading:2715671-Recombinant Proteins
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Production of monoclonal antibodies toward bovine interferons-alpha suitable for immunopurification.
|
| pubmed:affiliation |
Department of Biochemistry, Israel Institute for Biological Research, Ness-Ziona.
|
| pubmed:publicationType |
Journal Article
|