Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1989-5-24
pubmed:abstractText
Sl/Sld mutant mice are profoundly deficient in tissue mast cells as a result of a defect in the microenvironment promoting the development of these cells. To facilitate the analysis of the Sl mutation, we attempted to establish an in vitro system in which the in vivo defect of Sl/Sld mice could be reproduced. 3T3 cell lines were established from 17-day-old embryos of Sl/Sld and congenic +/+ genotypes and were cocultured with mast cells obtained in vitro from the bone marrow of +/+ mice. All eight 3T3 cell lines derived from +/+ of T-cell-derived growth factors. By contrast, none of eight 3T3 cell lines from Sl/Sld embryos supported mast cells under similar conditions. The defect in Sl/Sld 3T3 cells was further characterized as a failure to induce the G1-to-S transition in synchronized mast cells upon contact, suggesting that the Sl gene product is indispensable for this activity. When 3T3 cells of +/+ genotype, grown on pieces of cellulose acetate membrane, were transplanted into the peritoneal cavity of Sl/Sld mice, mast cells appeared locally in the transplanted 3T3 cell layers. These results suggested an essential role of fibroblasts in vivo as the tissue microenvironment promoting the development of mast cells and that they are defective in Sl/Sld mice. The present coculture system duplicated mast-cell deficiency of Sl/Sld mice in vitro and should prove useful for analysis of the Sl gene product.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-1083808, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-13985244, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-14440295, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-2871846, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-2937061, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-3086327, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-3275680, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-3401590, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-3491870, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-3551622, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-3580572, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-367470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-390999, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-4301006, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-4311790, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-5317869, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-561894, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-6230674, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-6510482, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-6809816, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-6972532, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-7026580, http://linkedlifedata.com/resource/pubmed/commentcorrection/2704751-7093523
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2888-91
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
In vitro duplication and in vivo cure of mast-cell deficiency of Sl/Sld mutant mice by cloned 3T3 fibroblasts.
pubmed:affiliation
Division of Cancer Pathology, Osaka University Medical School, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't