Linked Life Data

2686671

Source:http://linkedlifedata.com/resource/pubmed/id/2686671

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9-10
pubmed:dateCreated
1990-1-25
pubmed:abstractText
Although oxygen dependent photoreactions of 8-methoxypsoralen (8-MOP) are known, damage to DNA is mostly considered to proceed via photocycloaddition to this biomacromolecule. In this study the survival of colony forming ability of E. coli K12/343, which is deficient in DNA-repair capability, appeared to be lower in D2O than in H2O after exposure to the combination of 8-MOP and UV-A. Photobinding to bacterial DNA was approximately 40% higher in D2O than in H2O. However this last difference was found only when the bacteria were kept in the reaction medium for 1 h at 37 degrees C after the irradiation was stopped and not when they were plated out immediately afterwards. The results indicate that in this bacterial test system 1O2 mediated photobinding of 8-MOP to DNA contributes to the genotoxic effect observed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0939-5075
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
819-23
pubmed:dateRevised
2009-11-4
pubmed:meshHeading
pubmed:articleTitle
Singlet oxygen mediated photobinding of 8-methoxypsoralen to DNA and genotoxicity in E. coli.
pubmed:affiliation
Department of Pharmacochemistry, Leiden University, The Netherlands.
pubmed:publicationType
Journal Article