Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1989-12-8
pubmed:abstractText
We have developed a method for partially purifying chromatin from Saccharomyces cerevisiae (baker's yeast) to a level suitable for studies of its higher-order folding. This has required the use of yeast strains that are free of the ubiquitous yeast "killer" virus. Results from dynamic light scattering, electron microscopy, and x-ray diffraction show that the yeast chromatin undergoes a cation-dependent folding into 30-nm filaments that resemble those characteristic of higher-cell chromatin; moreover, the packing of nucleosomes within the yeast 30-nm filaments is similar to that of higher cells. These results imply that yeast has a protein or protein domain that serves the role of the histone H 1 found in higher cells; physical and genetic studies of the yeast activity could help elucidate the structure and function of H 1. Images of the yeast 30-nm filaments can be used to test crossed-linker models for 30-nm filament structure.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-1064861, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-10872332, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-2654891, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-2660830, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-3210247, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-334730, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-3733698, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-374341, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-3783706, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-3955173, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-4027333, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-4075395, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-4092681, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-6390339, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-6482966, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-6715407, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-7040415, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-7312631, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-7431398, http://linkedlifedata.com/resource/pubmed/commentcorrection/2682643-782917
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8266-70
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Higher-order structure of Saccharomyces cerevisiae chromatin.
pubmed:affiliation
Department of Chemistry, University of Illinois, Urbana 61801.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't