Linked Life Data

2674017

Source:http://linkedlifedata.com/resource/pubmed/id/2674017

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1989-10-23
pubmed:abstractText
A homologous polyclonal antibody was produced in a rabbit to the 104-kilodalton (kDa) protein hemolysin of Actinobacillus pleuropneumoniae serotype 1 strain CM-5. In immunoblots, this antibody recognized a similar 104-kDa protein produced in culture supernatants by A. pleuropneumoniae serotypes 1 to 12 and taxon "Minor group" in addition to Pasteurella haemolytica, Actinobacillus suis, and alpha-hemolysin-producing Escherichia coli (but only weakly in the latter two organisms). These results were reproduced by using a mouse monoclonal antibody to the CM-5 104-kDa protein hemolysin, except that the monoclonal antibody bound more strongly to the alpha-hemolysin produced by E. coli, only weakly to the 104-kDa protein produced by "Minor group," and not at all to any extracellular antigens produced by A. suis. Pigs experimentally infected with A. pleuropneumoniae serotypes 1 to 10 and A. suis produced an antibody that recognized the 104-kDa hemolysin produced by CM-5. A pig challenged with a "Minor group" strain did not have such antibodies. Rabbit antiserum produced against the leukotoxin of P. haemolytica and alpha-hemolysin-producing E. coli also recognized the CM-5 hemolysin, but the latter only weakly. The hemolytic activity produced by CM-5 in culture supernatant was neutralized strongly by the pig serum to serotypes 1, 2, 5, 6, 9, and 10 and A. suis, only partially by serotype 8 antiserum and the rabbit antiserum to P. haemolytica leukotoxin, and not all by the antiserum to serotypes 3, 4, and 7 and "Minor group" and the E. coli alpha-hemolysin. These results indicate that a similar but not identical 104-kDa protein is produced in vitro and in vivo by all serotypes of A. pleuropneumoniae and may be related to cytolysins produced by other gram-negative bacteria.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-2465272, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-2465275, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-2713790, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3417350, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3421527, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3514465, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3539296, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3592376, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3905610, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3943895, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3943899, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-3986682, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-6348003, http://linkedlifedata.com/resource/pubmed/commentcorrection/2674017-6493974
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3210-3
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in Actinobacillus pleuropneumoniae, Actinobacillus suis, Pasteurella haemolytica, and Escherichia coli.
pubmed:affiliation
Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't