Linked Life Data

2665839

Source:http://linkedlifedata.com/resource/pubmed/id/2665839

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-8-30
pubmed:abstractText
Recently, a human megakaryoblastic cell line, CMK, was established from the peripheral blood of a megakaryoblastic leukemia patient with Down syndrome. Using this cell line, we studied the proliferation and differentiation of megakaryocytic cells in the presence of highly purified human hematopoietic factors and phorbol 12-myristate-13-acetate (PMA). In a methylcellulose culture system, interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitated colony formation by CMK cells in a dose-dependent manner. The maximum stimulating doses of these factors were 10 and 200 U/mL, respectively. These concentrations were comparable to those that stimulate activity in normal hematopoietic cells. In contrast, granulocyte-colony stimulating factor (G-CSF), macrophage-colony stimulating factor (M-CSF), and erythropoietin (EPO) had no effects on the colony formation of CMK cells. In a liquid culture system, 20% of the CMK cells expressed glycoprotein IIb/IIIa (GPIIb/IIIa) antigen without hematopoietic factors, whereas 40% of the cells expressed GPIIb/IIIa with the addition of IL-3 and GM-CSF. EPO also slightly enhanced expression of GPIIb/IIIa. On the other hand, PMA inhibited growth of CMK cells and induced most of them to express the GPIIb/IIIa antigen. Furthermore, PMA induced CMK cells to produce growth activity toward new inocula of CMK cells. This growth factor (GF) contained colony-stimulating activity (CSA) in normal bone marrow (BM) cells. The activity was believed to be attributable mainly to GM-CSF, since 64% of this activity was neutralized by anti-GM-CSF antibodies and a transcript of GM-CSF was detected in mRNA from PMA-treated CMK cells by Northern blot analysis. These observations suggest that GM-CSF, as well as IL-3, should play an important role in megakaryocytopoiesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
42-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:2665839-Blotting, Northern, pubmed-meshheading:2665839-Bone Marrow Cells, pubmed-meshheading:2665839-Cell Differentiation, pubmed-meshheading:2665839-Cell Division, pubmed-meshheading:2665839-Colony-Stimulating Factors, pubmed-meshheading:2665839-Erythropoietin, pubmed-meshheading:2665839-Gene Expression Regulation, pubmed-meshheading:2665839-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:2665839-Growth Substances, pubmed-meshheading:2665839-Humans, pubmed-meshheading:2665839-Immunologic Techniques, pubmed-meshheading:2665839-Interleukin-1, pubmed-meshheading:2665839-Interleukin-3, pubmed-meshheading:2665839-Leukemia, Megakaryoblastic, Acute, pubmed-meshheading:2665839-Megakaryocytes, pubmed-meshheading:2665839-Platelet Membrane Glycoproteins, pubmed-meshheading:2665839-Tetradecanoylphorbol Acetate, pubmed-meshheading:2665839-Tumor Cells, Cultured
pubmed:year
1989
pubmed:articleTitle
Growth and differentiation of a human megakaryoblastic cell line, CMK.
pubmed:affiliation
Department of Medicine and Biochemistry II, Jichi Medical School, Tochigi, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't