|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1989-8-16
|
|
pubmed:abstractText |
It has previously been demonstrated that efficient DNA synthesis by oncogenic p21H-ras only occurs in the presence of insulin and is absolutely dependent on functional protein kinase C. Here we show that morphological transformation induced by oncogenic p21H-ras does not require functional protein kinase C. The early phases of protein kinase C-independent morphological transformation do not require de novo protein synthesis. We have also demonstrated that the introduction of p21H-ras into quiescent Swiss 3T3 cells by scrape-loading leads to increased levels of c-myc mRNA similar to those seen following serum stimulation. The increases in c-myc mRNA levels induced by p21H-ras are also independent of functional protein kinase C. Both morphological transformation and the elevation of c-myc mRNA levels do not require insulin. These results demonstrate that p21H-ras is generating protein kinase C-dependent and -independent signals.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2425352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2459146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2536916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2821624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2833702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2833817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2834200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2842690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-2842715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3000601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3001936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3018591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3018928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3023943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3027568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3090687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3104768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3278810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3283542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3302722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3313065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3323889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3550423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3918269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-3923359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6147754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6201494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6286693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6310338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6329026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6357812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6380758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6606489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2663469-6611509
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0261-4189
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
8
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1099-104
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2663469-Animals,
pubmed-meshheading:2663469-Cell Transformation, Neoplastic,
pubmed-meshheading:2663469-DNA,
pubmed-meshheading:2663469-Gene Expression Regulation,
pubmed-meshheading:2663469-Protein Biosynthesis,
pubmed-meshheading:2663469-Protein Kinase C,
pubmed-meshheading:2663469-Proto-Oncogene Proteins,
pubmed-meshheading:2663469-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:2663469-Proto-Oncogene Proteins p21(ras),
pubmed-meshheading:2663469-Proto-Oncogenes,
pubmed-meshheading:2663469-RNA, Messenger
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
p21H-ras-induced morphological transformation and increases in c-myc expression are independent of functional protein kinase C.
|
|
pubmed:affiliation |
Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
