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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1989-8-10
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pubmed:abstractText |
MPM-2, a monoclonal antibody specific for cells in mitosis, recognizes a family of proteins that share a common phosphorylated epitope. In this study we have shown that during the maturation of Xenopus laevis oocytes induced by progesterone, phosphorylation of MPM-2 antigens coincided with the appearance of MPF activity. When MPM-2 (0.7-1.4 micrograms per oocyte) was injected into oocytes prior to progesterone stimulation, MPF activity failed to appear and induction of maturation was inhibited as judged by both germinal-vesicle breakdown and white-spot formation. Further, MPM-2 was able to neutralize as well as immunodeplete MPF activity from mitotic HeLa cell and mature oocyte extracts. These results suggest that MPM-2 recognizes either MPF itself or a protein(s) that regulates MPF activity and that the kinase that phosphorylates MPM-2 antigens may be a key component in the regulation of M-phase induction.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-165088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-2674165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-2834064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-2901043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-3283736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-3290226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-3293802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-3293803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-3527814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-3539598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-3803713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-3821728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-5531072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-6266278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-6345556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-6352375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-6379644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-6424501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-6574461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2662192-885290
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4982-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2662192-Animals,
pubmed-meshheading:2662192-Antibodies, Monoclonal,
pubmed-meshheading:2662192-Antigen-Antibody Complex,
pubmed-meshheading:2662192-Female,
pubmed-meshheading:2662192-Growth Substances,
pubmed-meshheading:2662192-HeLa Cells,
pubmed-meshheading:2662192-Humans,
pubmed-meshheading:2662192-Kinetics,
pubmed-meshheading:2662192-Maturation-Promoting Factor,
pubmed-meshheading:2662192-Mitosis,
pubmed-meshheading:2662192-Oocytes,
pubmed-meshheading:2662192-Xenopus laevis
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pubmed:year |
1989
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pubmed:articleTitle |
Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte maturation and depletes maturation-promoting activity.
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pubmed:affiliation |
Department of Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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