Linked Life Data

265961

Source:http://linkedlifedata.com/resource/pubmed/id/265961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1977-6-30
pubmed:abstractText
The in vitro method for culture of molar teeth from eight-day-old rats, as reported in this study, appeared to sustain reasonably normal activity in the cells of the enamel organ and pulp through culture periods of four to eight hours. Inhibition of metabolic activity in the explants by addition of 5 mM iodoacetate or 2,4-dinitrophenol to the culture medium, or by heating at 70 C for 10 minutes, did not appear to affect the intensity or pattern of 45Ca uptake in the more advanced, rapidly mineralizing areas of the enamel. Neither did stripping of the enamel organ from the surface of the enamel have a demonstrable effect in those areas. However, metabolic inhibition with 2,4-dinitrophenol, heat killing or stripping of the enamel organ resulted in increased 45Ca uptake in newly formed enamel adjacent to the secreting ameloblasts. It is hypothesized that calcium flux into newly formed enamel matrix is controlled, in part, by movement of the calcium, which diffuses between the ameloblasts toward the enamel surface, away from the enamel through the ameloblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-0345
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
313-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
In vitro study of cellular influence on 45Ca uptake in developing rat enamel.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, P.H.S.