Linked Life Data

2654178

Source:http://linkedlifedata.com/resource/pubmed/id/2654178

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-6-22
pubmed:abstractText
In an initial evaluation, we found the Oxoid Signal blood culture system inferior to the BACTEC radiometric system for detection of some microorganisms causing septicemia (M. P. Weinstein, S. Mirrett, and L. B. Reller, J. Clin. Microbiol. 26:962-964, 1988). To determine whether modified processing of the Oxoid Signal blood culture system could improve its yield and speed of detecting positive cultures relative to the BACTEC radiometric system, we agitated all Oxoid bottles during the first 24 to 48 h of incubation and performed aerobic and anaerobic subcultures of all Oxoid bottles negative after 7 days of incubation. These modifications improved the overall performance of the Oxoid system, particularly with regard to the yield of streptococci, members of the family Enterobacteriaceae, and Haemophilus, Neisseria, and Acinetobacter spp. The speed of detecting positive cultures also was improved, especially within the first 24 h of incubation. However, the BACTEC system still detected more positive cultures (P less than 0.005), especially of obligate aerobes such as Pseudomonas aeruginosa (P less than 0.05) and yeasts (P less than 0.005). The BACTEC system also detected positive cultures earlier than the Oxoid system (e.g., at 24 h of incubation, 70.5% of BACTEC positive cultures detected versus 62.1% of Oxoid positive cultures detected). Further modifications of the Oxoid system which might include a revised medium, additional processing modifications, altered headspace atmosphere, or a complementary second broth medium should be considered, since the system is attractive in concept and is easy to use in the clinical laboratory.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-3098802, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-3384917, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-3530625, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-3546365, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-4124773, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-6415101, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-6828811, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-6999023, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-7021586, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-7031091, http://linkedlifedata.com/resource/pubmed/commentcorrection/2654178-7137116
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
427-30
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Effect of agitation and terminal subcultures on yield and speed of detection of the Oxoid Signal blood culture system versus the BACTEC radiometric system.
pubmed:affiliation
Microbiology Laboratory, Robert Wood Johnson University Hospital, New Brunswick, New Jersey 08901.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't