rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1989-3-15
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pubmed:abstractText |
Specific DNA probes were used to identify Shiga-like-toxin (SLT) I- and II-producing Escherichia coli from children less than 5 years of age with bloody diarrhea, in infants with diarrhea, and in controls of the same age without diarrhea in Thailand. At one hospital, SLT-producing E. coli was identified in 4 (7%) of 54 children with bloody diarrhea from whom other enteric pathogens were not identified and from 3 (6%) of 50 children without diarrhea. In the positive specimens, SLT-producing E. coli constituted only 0.3 to 4% of the 100 to 300 colonies on the replica blots. Non-toxin-encoding 933J and 933W bacteriophagelike DNA sequences were detected by colony hybridization with E. coli isolates from 18 (33%) of 54 children with bloody diarrhea and 23 (46%) of 50 controls. At another hospital, SLT-producing E. coli was not identified in 115 infants with diarrhea and 119 controls without diarrhea. One infant with diarrhea was infected with E. coli O76:H7 that hybridized with the enterohemorrhagic E. coli probe but not with the SLT probes. E. coli producing SLT I or SLT II was isolated in small numbers from a similar proportion of Thai children with bloody diarrhea in whom no other enteric pathogen was identified and from controls without diarrhea.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2644294-1055375,
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Feb
|
pubmed:issn |
0095-1137
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
27
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
291-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2644294-Bacterial Toxins,
pubmed-meshheading:2644294-Child, Preschool,
pubmed-meshheading:2644294-DNA, Bacterial,
pubmed-meshheading:2644294-DNA Probes,
pubmed-meshheading:2644294-Diarrhea, Infantile,
pubmed-meshheading:2644294-Escherichia coli,
pubmed-meshheading:2644294-Female,
pubmed-meshheading:2644294-Humans,
pubmed-meshheading:2644294-Infant,
pubmed-meshheading:2644294-Male,
pubmed-meshheading:2644294-Nucleic Acid Hybridization,
pubmed-meshheading:2644294-Shiga Toxins,
pubmed-meshheading:2644294-Thailand
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pubmed:year |
1989
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pubmed:articleTitle |
Determination by DNA hybridization of Shiga-like-toxin-producing Escherichia coli in children with diarrhea in Thailand.
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pubmed:affiliation |
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
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pubmed:publicationType |
Journal Article
|