Linked Life Data

2625035

Source:http://linkedlifedata.com/resource/pubmed/id/2625035

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-4-6
pubmed:abstractText
The Wampole Bactigen Salmonella-Shigella Latex Agglutination Test (SSLA) (Wampole Laboratories, Cranbury, New Jersey) was evaluated as a possible substitute for blind subculture of selenite broths from stool cultures. Recovery rates of Salmonella and Shigella from eosin-methylene blue (EMB) agar were reviewed to determine if this medium could be eliminated from primary stool culture. Salmonella was detected in 17 of 822 stools by both SSLA and culture. There were 52 false-positive SSLA for Salmonella (sensitivity 100%, specificity 93%). Of three Shigella isolated on culture, one was SSLA positive, one was SSLA negative, and one was negative by both SSLA and subculture of selenite broth. There were eight false-positive SSLA for Shigella (specificity 99%). Of 50 Salmonella and 11 Shigella isolated from 6200 stools in 1.5 years, two Shigella were isolated on EMB only. The SSLA test is a useful screening test for Salmonella. By eliminating unnecessary subcultures of selenite broth, it reduces turnaround time by 24 hr for negative stool cultures. The combination of primary culture with SSLA screening of enrichment broth should be adequate for the detection of Salmonella and Shigella from stool specimens. Our data suggest that EMB or other differential medium should be retained for primary culture to enhance detection of Shigella.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0732-8893
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
463-6
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:articleTitle
Increased efficiency of stool culture for the detection of Salmonella and Shigella.
pubmed:affiliation
Section of Clinical Microbiology, Mayo Clinic, Rochester, MN 55905.
pubmed:publicationType
Journal Article