Linked Life Data

2621062

Source:http://linkedlifedata.com/resource/pubmed/id/2621062

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-3-22
pubmed:abstractText
A series of monoclonal antibodies was prepared against purified adenovirus type 2 fiber. These antibodies were produced by both classical techniques and by electrofusion. Fifty antibodies were obtained, and five were selected which had the following characteristics: (i) they were able to reduce adenovirus 2 infection of Hep-2 cells; (ii) they had different light-chain structures and (iii) they did not react with denatured fiber. The five antibodies (AdF18, AdF38, AdF41, AdF2D3, AdF3B1) recognized between them three periodate-insensitive epitopes on the fiber. AdF18 bound to both adenovirus type 2 and type 5; all the others were specific to type 2. Polyclonal anti-idiotype antibodies to these antibodies were produced in rabbits and were purified extensively on immunoaffinity columns. Anti-idiotypes to AdF38 and AdF41 were able to inhibit virus infectivity, and anti-AdF41 completed with fiber for binding to the surface of Hep-2 cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-5526
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
313-22
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Anti-idiotype response to selected neutralizing monoclonal antibodies generated against adenovirus type 2 fiber.
pubmed:affiliation
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Québ., Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't