|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
10
|
|
pubmed:dateCreated |
1990-1-9
|
|
pubmed:abstractText |
We measured isotype-specific human antibodies directed against Campylobacter jejuni native flagellin and a synthetic peptide derived from the N-terminal amino acid sequence of the protein by using a microdilution enzyme-linked immunosorbent assay (ELISA). Serum samples from patients with gastrointestinal infection caused by C. jejuni (n = 20) and control samples (number from normal subjects = 20; number from patients with diarrhea other than campylobacter = 20) were tested in this assay. Serum specimens from patients with campylobacter infection showed statistically significant higher isotype-specific antiflagellin antibody titers than control samples did. Detection of immunoglobulin G (IgG) antibodies was less specific (70%) than detection of either IgA or IgM antibodies in infected patients (95%). The sensitivity of testing for any of the isotypes ranged from 64 to 100% in acute-phase serum specimens and 85 to 95% in convalescent-phase serum specimens. An ELISA with an N-terminal synthetic peptide derived from the flagellin protein as antigen was not sensitive (60%) for detecting campylobacter infection but was very specific (97.5%). In conclusion, detection of serum IgA or IgM against C. jejuni flagellin may be a useful marker of infection. Although the N-terminal synthetic peptide was antigenic in a few patients with infection and showed good specificity in the ELISA, additional amino acid sequences with better sensitivity for detecting infection need to be identified.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-2444578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-2444623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-2578478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-2578479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-2873103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-3137040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-3611318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-4056739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-6715034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-6885981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2584372-708370
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0095-1137
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
27
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2195-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2584372-Amino Acid Sequence,
pubmed-meshheading:2584372-Antibodies, Bacterial,
pubmed-meshheading:2584372-Antigens, Bacterial,
pubmed-meshheading:2584372-Bacterial Proteins,
pubmed-meshheading:2584372-Binding, Competitive,
pubmed-meshheading:2584372-Campylobacter fetus,
pubmed-meshheading:2584372-Flagellin,
pubmed-meshheading:2584372-Humans,
pubmed-meshheading:2584372-Immunoglobulin A,
pubmed-meshheading:2584372-Immunoglobulin G,
pubmed-meshheading:2584372-Immunoglobulin M,
pubmed-meshheading:2584372-Molecular Sequence Data,
pubmed-meshheading:2584372-Peptides
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Human antibody response to Campylobacter jejuni flagellin protein and a synthetic N-terminal flagellin peptide.
|
|
pubmed:affiliation |
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-4283.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
