Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-3-1
pubmed:abstractText
We studied the regulation of in vivo expression of Escherichia coli glutaminyl-tRNA synthetase at the transcriptional and translational level by analysis of glnS mRNA and glutaminyl-tRNA synthetase levels under a variety of growth conditions. In addition, strains carrying fusions of the beta-galactosidase structural gene and the glnS promoter were constructed and subsequently used for glnS regulatory studies. The level of glutaminyl-tRNA synthetase increases with the increasing growth rate, with a concomitant though much larger increase in glnS mRNA levels. Thus, transcriptional control appears to mediate metabolic regulation. It is known that glnR5, a regulatory mutation unlinked to glnS, causes overproduction of glutaminyl-tRNA synthetase. Here we showed that the glnR5 product enhances transcription of glnS 10- to 15-fold. The glnR5 mutation does not affect metabolic control. Thus, glnS appears to be regulated by two different control systems affecting transcription. Furthermore, our results suggest post-transcriptional regulation of glutaminyl-tRNA synthetase.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-1097418, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-1180514, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-177401, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-24122, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-318645, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-342703, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-35231, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-353304, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-358278, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-375009, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-378953, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-378954, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-4568765, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-4570769, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-4592258, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-4612020, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-4618855, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-4902142, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-4906906, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6086662, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6162838, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6264314, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6288695, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6308658, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6317865, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6317866, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6325425, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6793240, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-6997493, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-7027258, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-7047500, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-922889, http://linkedlifedata.com/resource/pubmed/commentcorrection/2578447-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
212-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Two control systems modulate the level of glutaminyl-tRNA synthetase in Escherichia coli.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.