Linked Life Data

2574223

Source:http://linkedlifedata.com/resource/pubmed/id/2574223

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-1-25
pubmed:abstractText
The effect of gamma-irradiation of pSV2gpt DNA on its transfection frequency has been analysed using CHO xrs mutants. Xrs mutants are sensitive to ionizing radiation and show a defect in double-strand break (dsb) rejoining. At low doses a sharp decrease in relative transfection frequency, i.e. transfection frequency of irradiated plasmid relative to untreated plasmid, was observed in the xrs mutants compared with the parent line K1. Electrophoresis of the irradiated plasmid DNA showed that the decrease in transfection frequency in the xrs mutants correlated with the change of supercoiled molecules into open-circular forms. One explanation for these results is that the xrs gene could play a part in the integration or repair of open-circular molecules produced by gamma-radiation. In the parent line CHO-K1, open-circular and supercoiled molecules have the same transfection frequency. The effect of linearization of pSV2gpt DNA by restriction enzymes on transfection frequency in xrs and wild-type strains has also been examined. In contrast to the above results we have not detected a difference in the relative transfection frequency between xrs and wild-type strains. The results suggest that restricted plasmid DNA is subject to extensive nucleolytic degradation, and this occurs to equal extents in wild type and mutant strains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0955-3002
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
951-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Use of damaged plasmid to study DNA repair in X-ray sensitive (xrs) strains of Chinese hamster ovary (CHO) cells.
pubmed:affiliation
Genetics Division, National Institute for Medical Research, London, U.K.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't