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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017638,
umls-concept:C0020200,
umls-concept:C0020205,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0027819,
umls-concept:C0039062,
umls-concept:C0205464,
umls-concept:C0871261,
umls-concept:C1522642,
umls-concept:C1704336,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1709059,
umls-concept:C2911692
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pubmed:issue |
2
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pubmed:dateCreated |
1978-7-24
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|
pubmed:abstractText |
Cells of the hybrid clone NG108-15 responded to 5-hydroxytryptamine (5-HT), dopamine or acetylcholine with graded depolarizations involving membrane conductance increases. Responses desensitized during continuous application of the neurotransmitters, and responses to 5-HT and dopamine cross-desensitized: a desensitizing application of one neurotransmitter also desensitized the hybrd cell to the other neurotransmitter. 5-HT and acetylcholine did not cross-desensitize. The hybrid cell 5-HT response was not attenuated by D-LSD, and was blocked by 10(-5) M morphine, although not via binding to naloxone-sensitive opiate receptors. 5-HT or the prostaglandin PGF2alpha caused the release of acetylcholine at the synapses of hybrid cells with mouse myotubes. Application of 5-HT or PGF2alpha also facilitated the synaptic release elicited by hybrid cell action potentials. Following treatment with the antimitotic agent cytosine arabinoside, co-cultures of hybrid cells and mouse myotubes exhibited plentiful synaptic connections only if maintained in medium containing 1 mM dibutyryl cAMP (dBcAMP). After X-irradiation, co-cultures were synaptically active even in the absence of dBcAMP. Thus, methods have been found to regulate both the short-term and long-term synaptic activity of NG108-15 hybrid cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-8993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
147
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
261-76
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:25697-Acetylcholine,
pubmed-meshheading:25697-Animals,
pubmed-meshheading:25697-Bucladesine,
pubmed-meshheading:25697-Clone Cells,
pubmed-meshheading:25697-Dopamine,
pubmed-meshheading:25697-Glioma,
pubmed-meshheading:25697-Membrane Potentials,
pubmed-meshheading:25697-Mice,
pubmed-meshheading:25697-Morphine,
pubmed-meshheading:25697-Neoplasms, Experimental,
pubmed-meshheading:25697-Neural Conduction,
pubmed-meshheading:25697-Neuroblastoma,
pubmed-meshheading:25697-Neuromuscular Junction,
pubmed-meshheading:25697-Neurotransmitter Agents,
pubmed-meshheading:25697-Prostaglandins F,
pubmed-meshheading:25697-Receptors, Opioid,
pubmed-meshheading:25697-Serotonin,
pubmed-meshheading:25697-Synapses
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pubmed:year |
1978
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pubmed:articleTitle |
Pharmacologic responses of cells of a neuroblastoma X glioma hybrid clone and modulation of synapses between hybrid cells and mouse myotubes.
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pubmed:publicationType |
Journal Article
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