Linked Life Data

2565334

Source:http://linkedlifedata.com/resource/pubmed/id/2565334

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-6-7
pubmed:abstractText
In Escherichia coli, mutations in arcA (dye) or arcB anaerobically derepress the synthesis of a multitude of enzymes of aerobic function, and mutations in arcA or cpxA impair F-pilus formation. It is thought that arcA encodes a promoter-recognizing protein, whereas arcB and cpxA encode sensor proteins which interact with the arcA product. In this study we found that anaerobic growth of a wild-type F' strain decreased the synthesis of both the enzymes and the pilus. Although the two arcA mutants examined were both anaerobically derepressed in the enzymes and impaired in aerobic pilus formation as expected, one mutant hyperproduced the pilus anaerobically. The two arcB mutants examined showed normal pilus formation when grown aerobically. When grown anaerobically they developed more pili than the wild-type strain did when grown aerobically. When a cpxA mutant was examined for synthesis of two aerobic enzymes, normal regulation was found. The available data suggest the following. The arcA product anaerobically represses certain genes of aerobic function and activates certain genes related to F function. It appears that the arcB product senses the redox or energy state; absence of the gene function shifts the arcA product to the nonrepressive form for enzyme synthesis for aerobic pathways. The cpxA product, on the other hand, senses the sexual state; absence of the gene function shifts the arcA product to the inactive form for F-pilus synthesis.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
171
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2889-93
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Differentiation of arcA, arcB, and cpxA mutant phenotypes of Escherichia coli by sex pilus formation and enzyme regulation.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.