Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-3-21
pubmed:abstractText
The human genome contains approximately 50,000 copies of an interspersed repeat with the sequence (dT-dG)n, where n = approximately 10-60. In humans, (TG)n repeats have been found in several sequenced regions. Since minisatellite regions with larger repeat elements often display extensive length polymorphisms, we suspected that (TG)n repeats ("microsatellites") might also be polymorphic. Using the polymerase chain reaction to amplify a (TG)n microsatellite in the human cardiac actin gene, we detected 12 different allelic fragments in 37 unrelated individuals, 32 of whom were heterozygous. Codominant Mendelian inheritance of fragments was observed in three families with a total of 24 children. Because of the widespread distribution of (TG)n microsatellites, polymorphisms of this type may be generally abundant and present in regions where minisatellites are rare, making such microsatellite loci very useful for linkage studies in humans.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-1105573, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-13092734, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-2448875, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-2851536, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-2994065, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-3029872, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-3282981, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-3422543, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-3540602, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-3856104, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-6142531, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-6247908, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-6273813, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-6310553, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-6326095, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-6584914, http://linkedlifedata.com/resource/pubmed/commentcorrection/2563634-728996
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0002-9297
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
397-401
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene.
pubmed:affiliation
Department of Biochemistry, Oregon Health Sciences University, Portland 97201.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.