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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-1-8
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pubmed:abstractText |
The effects of the muscarinic agonist carbachol, histamine and bradykinin on incorporation of [3H]inositol into the phosphoinositides and the formation of [3H]InsPs were examined in bovine tracheal smooth-muscle (BTSM) slices labelled with [3H]inositol. These agonists result in substantial and dose-related increases in the incorporation of [3H]inositol into the phospholipids. Carbachol and histamine stimulated the incorporation of [3H]inositol into the phospholipids to the same degree, despite histamine being only 35% as effective as carbachol on [3H]InsP accumulation. Histamine and carbachol, at maximal concentrations, were non-additive with respect to both the stimulated incorporation of [3H]inositol and [3H]InsP formation. For carbachol this effect on incorporation was found to occur to a similar extent in PtdInsP and PtdInsP2 as well as PtdIns. The initial effect of carbachol on [3H]inositol incorporation was rapid (maximal by 10 min); however, with prolonged stimulation large secondary declines in PtdInsP and PtdInsP2 labelling were observed, with depletion of the much larger PtdIns pool only evident in the presence of Li+. Lowering buffer [Ca2+] increased the incorporation of [3H]inositol under basal conditions, but did not attenuate the subsequent agonist-stimulated incorporation effect. The large changes in specific radioactivity of the phosphoinositides, and consequently the [3H]InsP products, after carbachol stimulation resulted in the apparent failure of atropine to reverse the [3H]InsP response completely. Labelling muscle slices with [3H]inositol in the presence of carbachol or labelling for longer periods (greater than 6 h) prevented subsequent carbachol-stimulated effects on incorporation without significantly altering the dose-response relationship for carbachol-stimulated [3H]InsP formation and resulted in steady-state labelling conditions confirmed by the ability of atropine to reverse fully the [3H]InsP response to carbachol. This study demonstrates the profound effects of a number of agonists on [3H]inositol incorporation into the phospho- and polyphosphoinositides in BTSM with important consequent changes in the specific radioactivity of these lipids and the resulting [3H]InsP products. In addition, a selective depletion of PtdInsP and PtdInsP2 over PtdIns has been demonstrated with prolonged muscarinic-receptor stimulation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-1267780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-13084667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-164246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-18462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-217364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-2417230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-2432219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-2452969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-2725701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-2849446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-2905910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-2906146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-2981282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-3014119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-3021749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-3028426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-3304132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-3707540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-3876861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-4377209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6157980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6194786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6259001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6320795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6323632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6323902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6330778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6423773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-6593735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-7037762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2556108-7150264
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
262
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
739-46
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2556108-Animals,
pubmed-meshheading:2556108-Bradykinin,
pubmed-meshheading:2556108-Carbachol,
pubmed-meshheading:2556108-Cattle,
pubmed-meshheading:2556108-Dose-Response Relationship, Drug,
pubmed-meshheading:2556108-Histamine,
pubmed-meshheading:2556108-Inositol,
pubmed-meshheading:2556108-Inositol Phosphates,
pubmed-meshheading:2556108-Muscle, Smooth,
pubmed-meshheading:2556108-Phosphatidylinositols,
pubmed-meshheading:2556108-Stimulation, Chemical,
pubmed-meshheading:2556108-Time Factors,
pubmed-meshheading:2556108-Trachea,
pubmed-meshheading:2556108-Tritium
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pubmed:year |
1989
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pubmed:articleTitle |
Characterization of agonist-stimulated incorporation of myo-[3H]inositol into inositol phospholipids and [3H]inositol phosphate formation in tracheal smooth muscle.
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pubmed:affiliation |
Department of Pharmacology and Therapeutics, University of Leicester, U.K.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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