Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1990-1-8
pubmed:abstractText
We describe the mapping and sequencing of mutations within the DNA polymerase gene of herpes simplex virus type 1 which confer resistance to aphidicolin, a DNA polymerase inhibitor. The mutations occur near two regions which are highly conserved among DNA polymerases related to the herpes simplex enzyme. They also occur near other herpes simplex mutations which affect the interactions between the polymerase and deoxyribonucleoside triphosphate substrates. Consequently, we argue in favor of the idea that the aphidicolin binding site overlaps the substrate binding site and that the near-by conserved regions are functionally required for substrate binding. Our mutants also exhibit abnormal sensitivity to another DNA polymerase inhibitor, phosphonoacetic acid. This drug is thought to bind as an analogue of pyrophosphate. A second-site mutation which suppresses the hypersensitivity of one mutant to phosphonoacetic acid (but not its aphidicolin resistance) is described. This second mutation may represent a new class of mutations, which specifically affects pyrophosphate, but not substrate, binding.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-16453611, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-232215, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2458565, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2542598, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2670563, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2832946, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2842788, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2987953, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2991710, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2999714, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-2999787, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3012524, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3015009, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3017656, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3018124, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3023690, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3027369, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3029408, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3034575, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3059678, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3072719, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3090778, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3127204, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3162770, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3194400, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3273407, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3286635, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3287376, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3322943, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3359994, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3383003, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-3479792, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-354496, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-482131, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-55273, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-592361, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6087149, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6092371, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6167579, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6243429, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6250617, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6250618, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6271750, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6276402, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6280605, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6286645, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6308578, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6320535, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6328755, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6402775, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6473099, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6784928, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-6809534, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-7142161, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-7356466, http://linkedlifedata.com/resource/pubmed/commentcorrection/2555788-88745
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9231-44
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
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