Linked Life Data

2554237

Source:http://linkedlifedata.com/resource/pubmed/id/2554237

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1989-12-21
pubmed:abstractText
SV40 large T antigen interacts specifically with three different DNA sequences within the SV40 control region namely site I, II and III, in order to regulate the viral DNA replication and the stimulation of late, or repression of early viral transcription. We define three different plasmids, containing either site I alone, site II with SV40 specific flanking sequences or both sites I and II with additional neighbouring sequences which were used to study the interaction of T antigen with each of its binding sites. We found that binding of T antigen to site II reduced binding of T antigen to site I as opposed to the interaction of T antigen with site I alone. These results were supported by our findings that T antigen from an SV40 temperature-sensitive mutant, tsA58, which is heat-sensitive for binding to site II, exhibited the same binding affinity for site I as for the DNA fragment containing both binding sites. Thus, binding of T antigen to binding site II seems to interfere with binding to site I, thereby influencing the regulatory functions of T antigen in the repression of early viral gene transcription.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1353-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Complex interaction of SV40 large T antigen with the control region on the SV40 DNA.
pubmed:affiliation
Department of Biochemistry, University of Ulm, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't