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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-12-4
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pubmed:abstractText |
We studied regulation of human immunodeficiency virus-1 (HIV-1) transcription by Tat and, for comparative purposes, by the adenovirus E1A protein. These two trans-activators exerted different effects. Two classes of HIV-1-promoted cytoplasmic RNA were detected, one class corresponding to full-length transcripts and the other to transcripts ending 55 and 59 nucleotides from the transcription start. Tat increased the level of the full-length class only, whereas E1A increased the levels of both classes of RNA. We also measured the effects of Tat and E1A on RNA synthesis rates. Without trans-activators, HIV-1-directed transcription was relatively weak and exhibited a marked polarity. Both Tat and E1A dramatically increased promoter-proximal transcription, while only Tat suppressed transcriptional polarity. Mutations in the TAR element did not influence basal transcription rates or the response to E1A, but eliminated trans-activation by Tat. We propose that Tat acts through TAR to increase initiation complex formation on the HIV-1 promoter and to stabilize complexes during elongation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed RNA Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
283-92
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2553266-Adenovirus Early Proteins,
pubmed-meshheading:2553266-Adenoviruses, Human,
pubmed-meshheading:2553266-DNA-Binding Proteins,
pubmed-meshheading:2553266-DNA-Directed RNA Polymerases,
pubmed-meshheading:2553266-Gene Expression,
pubmed-meshheading:2553266-Gene Products, tat,
pubmed-meshheading:2553266-Genes, Viral,
pubmed-meshheading:2553266-HIV-1,
pubmed-meshheading:2553266-HeLa Cells,
pubmed-meshheading:2553266-Humans,
pubmed-meshheading:2553266-Models, Genetic,
pubmed-meshheading:2553266-Mutation,
pubmed-meshheading:2553266-Oncogene Proteins, Viral,
pubmed-meshheading:2553266-Plasmids,
pubmed-meshheading:2553266-RNA, Viral,
pubmed-meshheading:2553266-Restriction Mapping,
pubmed-meshheading:2553266-Trans-Activators,
pubmed-meshheading:2553266-Transcription, Genetic,
pubmed-meshheading:2553266-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1989
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pubmed:articleTitle |
HIV-1 Tat protein increases transcriptional initiation and stabilizes elongation.
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pubmed:affiliation |
Cold Spring Harbor Laboratory, New York 11724.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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