Linked Life Data

2547837

Source:http://linkedlifedata.com/resource/pubmed/id/2547837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-9-21
pubmed:abstractText
Endothelins are a group of potent vasoconstrictors whose structure was deduced from genomic DNA. ET-1 was first isolated from culture supernatants from porcine endothelial cells and ET-3 was identified from a rat DNA library. We report on the binding of 125I-ET-1 to zona glomerulosa cells in culture and on its ability to stimulate aldosterone secretion. Cultured calf adrenal zona glomerulosa cells have saturable, high affinity [Kd = 1.00 +/- 0.17 X 10(-10) M (SEM)] receptors which bind ET-1 in a temperature and time dependent manner. Binding was specific and angiotensin II, vasopressin, ANP, BNP, apamin, calcium channel agonists or antagonists did not interact with the receptor. ET-3 displaced 125I-ET-1 from the receptor with a relative potency of 0.39 +/- 0.1% (SEM) that of ET-1. ET-1 incubated with cultured glomerulosa cells stimulated aldosterone secretion in a dose dependent manner but it was less potent than angiotensin II. ET-3 had less than 1% the relative potency of ET-1 stimulating aldosterone secretion. This data suggest that ET-1 is an independent stimulator of aldosterone secretion and we are speculating that it might be important in those situations, like in malignant hypertension, where endothelial damage might result in increased ET-1 production.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2451132, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2460098, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2462875, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2536280, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2538185, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2642921, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2643430, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2649896, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-2849431, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-3044368, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-3045827, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-3052450, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-3059828, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-3282927, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-3314460, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-3453564, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-3993773, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-5568230, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-6253831, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-6284482, http://linkedlifedata.com/resource/pubmed/commentcorrection/2547837-6882371
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1032-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Endothelin binding to cultured calf adrenal zona glomerulosa cells and stimulation of aldosterone secretion.
pubmed:affiliation
Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.