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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0014644,
umls-concept:C0017262,
umls-concept:C0025252,
umls-concept:C0205275,
umls-concept:C0441889,
umls-concept:C1171362,
umls-concept:C1407029,
umls-concept:C1510411,
umls-concept:C1514562,
umls-concept:C1515670,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
6
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pubmed:dateCreated |
1989-6-27
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pubmed:abstractText |
A previously unrecognized activity has been associated with the product of the BNLF-1 gene of Epstein-Barr virus. This gene encodes the latent membrane protein of Epstein-Barr virus. When the gene was expressed at high levels, it was toxic to all cell lines tested, which included six human B-lymphoid lines as well as BALB/3T3, 143/EBNA-1, and HEp-2 cells. The BNLF-1 gene was previously shown to induce anchorage-independent and tumorigenic growth in Rat-1 and BALB/3T3 cells. We demonstrate here that only those mutations in the BNLF-1 gene that score positively in the anchorage-independent growth assay were cytotoxic when expressed at high levels. It is therefore possible that the same activities of the latent membrane protein that are necessary to induce anchorage-independent growth of some rodent cell lines also confer toxicity to many cell lines when expressed at high levels.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-14304234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-205793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2536919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2823633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2824192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2836780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2838956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2983194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2983224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2991567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-2991591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-3000618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-3023199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-3025615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-3027413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-3288862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6086953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6087149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6094955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6095274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6098817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6101202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6276755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6283119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6300444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2542565-6933474
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2469-75
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2542565-Animals,
pubmed-meshheading:2542565-Antigens, Viral,
pubmed-meshheading:2542565-Cell Line,
pubmed-meshheading:2542565-Cell Transformation, Viral,
pubmed-meshheading:2542565-Enhancer Elements, Genetic,
pubmed-meshheading:2542565-Gene Expression Regulation,
pubmed-meshheading:2542565-Herpesvirus 4, Human,
pubmed-meshheading:2542565-Mutation,
pubmed-meshheading:2542565-Plasmids,
pubmed-meshheading:2542565-Promoter Regions, Genetic,
pubmed-meshheading:2542565-Restriction Mapping,
pubmed-meshheading:2542565-Viral Matrix Proteins
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pubmed:year |
1989
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pubmed:articleTitle |
The transforming domain alone of the latent membrane protein of Epstein-Barr virus is toxic to cells when expressed at high levels.
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pubmed:affiliation |
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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