Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6218
pubmed:dateCreated
1989-6-9
pubmed:abstractText
Telomeres confer stability on chromosomes by protecting them from degradation and recombination and by allowing complete replication of the end. They are genetically important as they define the ends of the linkage map. Telomeres of lower eukaryotes contain short repeats consisting of a G-rich and a C-rich strand, the G-rich strand running 5'-3' towards the telomere and extending at the end. Telomeres of human chromosomes share characteristics with those of lower eukaryotes including sequence similarity as detected by cross-hybridization. Telomeric repeats from many organisms can provide telomere function in yeast. Here we describe a modified yeast artificial chromosome (YAC) vector with only one telomere which we used to clone human telomeres by complementation in yeast. YACs containing human telomeres were identified by hydridization to an oligonucleotide of the trypanosome telomeric repeat. A subcloned human fragment from one such YAC is immediately subtelomeric on at least one human chromosome.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
338
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
771-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2541341-Animals, pubmed-meshheading:2541341-Chromosomes, Fungal, pubmed-meshheading:2541341-Chromosomes, Human, pubmed-meshheading:2541341-Cloning, Molecular, pubmed-meshheading:2541341-DNA, pubmed-meshheading:2541341-DNA Restriction Enzymes, pubmed-meshheading:2541341-Genes, Fungal, pubmed-meshheading:2541341-Genetic Markers, pubmed-meshheading:2541341-Genetic Vectors, pubmed-meshheading:2541341-Humans, pubmed-meshheading:2541341-Male, pubmed-meshheading:2541341-Nucleic Acid Hybridization, pubmed-meshheading:2541341-Oligonucleotide Probes, pubmed-meshheading:2541341-Recombination, Genetic, pubmed-meshheading:2541341-Saccharomyces cerevisiae, pubmed-meshheading:2541341-Sequence Homology, Nucleic Acid, pubmed-meshheading:2541341-Spermatozoa, pubmed-meshheading:2541341-Transformation, Genetic, pubmed-meshheading:2541341-Trypanosoma
pubmed:year
1989
pubmed:articleTitle
Cloning of human telomeres by complementation in yeast.
pubmed:affiliation
MRC Human Genetics Unit, Western General Hospital, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't