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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1989-6-22
|
| pubmed:abstractText |
Multiple forms of the catalytic subunit of the (Na+,K+)ATPase have been identified in rat brain. While two of them (alpha 1 and alpha 2) have been well characterized, the third form (alpha 3) of these catalytic subunits only recently has been described by cDNA cloning; the corresponding polypeptide has not been isolated. In this paper it is shown that rat brain contains the alpha 3 chain. The catalytic subunits of the (Na+, K+)ATPase from rat brain axolemma were purified by SDS-PAGE and subjected to formic acid cleavage. Amino acid sequence analysis of the resulting fragments revealed that axolemma has the alpha 3 form of the catalytic subunit. In addition, alpha 3-specific antiserum was raised in rabbits immunized with a synthetic peptide. Immunoblotting with this antiserum revealed that the alpha 3 form of the (Na+,K+)ATPase is present also in whole brain microsomes. In SDS-PAGE, the mobilities of the three catalytic subunits of brain (Na+, K+)ATPase follow the order alpha 1 greater than alpha 2 greater than alpha 3. Determination of the ouabain-inhibitable ATPase activity indicates that if the alpha 3 form of the (Na+,K+)ATPase is able to hydrolyze ATP, it is present in a form of the enzyme with a high affinity for this cardiac glycoside and is similar to the alpha 2 form in this respect.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0006-2960
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
28
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
569-73
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2540801-Amino Acid Sequence,
pubmed-meshheading:2540801-Animals,
pubmed-meshheading:2540801-Brain,
pubmed-meshheading:2540801-Cell Membrane,
pubmed-meshheading:2540801-Immunoblotting,
pubmed-meshheading:2540801-Intracellular Membranes,
pubmed-meshheading:2540801-Isoenzymes,
pubmed-meshheading:2540801-Kidney,
pubmed-meshheading:2540801-Macromolecular Substances,
pubmed-meshheading:2540801-Male,
pubmed-meshheading:2540801-Microsomes,
pubmed-meshheading:2540801-Molecular Sequence Data,
pubmed-meshheading:2540801-Muscles,
pubmed-meshheading:2540801-Organ Specificity,
pubmed-meshheading:2540801-Ouabain,
pubmed-meshheading:2540801-Rats,
pubmed-meshheading:2540801-Rats, Inbred Strains,
pubmed-meshheading:2540801-Sodium-Potassium-Exchanging ATPase
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Rat brain has the alpha 3 form of the (Na+,K+)ATPase.
|
| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|