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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-4-25
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pubmed:abstractText |
The interaction of insulin-like growth factor (IGF)-I and IGF-II with specific type-I and -II receptor sites on rabbit articular chondrocyte membranes was studied. With labelled IGF-I as tracer, half-maximal displacement of the label was obtained with 1.4 ng IGF-I/ml and 22 ng IGF-II/ml. Using IGF-II as labelled peptide. 16 ng unlabelled IGF-II/ml and 200 ng IGF-I/ml were needed to inhibit the binding by 50%. Covalent cross-linking experiments revealed the presence of typical type-I (Mr 130,000 under reducing conditions) and type-II (Mr 260,000) receptor sites. In addition, with 125I-labelled IGF-II a very intense labelled band appeared at Mr greater than 300,000. This band was not found in mouse liver membranes and human placental membranes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-0795
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
120
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
245-9
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:2538534-Animals,
pubmed-meshheading:2538534-Binding, Competitive,
pubmed-meshheading:2538534-Cartilage, Articular,
pubmed-meshheading:2538534-Cell Membrane,
pubmed-meshheading:2538534-Cells, Cultured,
pubmed-meshheading:2538534-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2538534-Female,
pubmed-meshheading:2538534-Insulin-Like Growth Factor I,
pubmed-meshheading:2538534-Insulin-Like Growth Factor II,
pubmed-meshheading:2538534-Molecular Weight,
pubmed-meshheading:2538534-Rabbits,
pubmed-meshheading:2538534-Receptors, Cell Surface,
pubmed-meshheading:2538534-Receptors, Somatomedin
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pubmed:year |
1989
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pubmed:articleTitle |
Characterization of specific insulin-like growth factor (IGF)-I and IGF-II receptors on cultured rabbit articular chondrocyte membranes.
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pubmed:affiliation |
University Hospital for Children and Youth, 'Het Wilhelmina Kinderziekenhuis', State University of Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article
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