| pubmed-article:2537951 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2537951 | lifeskim:mentions | umls-concept:C1330957 | lld:lifeskim |
| pubmed-article:2537951 | lifeskim:mentions | umls-concept:C0012906 | lld:lifeskim |
| pubmed-article:2537951 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:2537951 | lifeskim:mentions | umls-concept:C0332161 | lld:lifeskim |
| pubmed-article:2537951 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:2537951 | lifeskim:mentions | umls-concept:C0007996 | lld:lifeskim |
| pubmed-article:2537951 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:2537951 | lifeskim:mentions | umls-concept:C0596311 | lld:lifeskim |
| pubmed-article:2537951 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2537951 | pubmed:dateCreated | 1989-4-12 | lld:pubmed |
| pubmed-article:2537951 | pubmed:abstractText | Two DNA duplexes of identical sequence and 35 nt in length were synthesized by an original and a highly improved version of phosphoramidite chemistry. By base composition analysis, DNA synthesized by improved chemistry (termed DMTS-imp) contained no detectable modified bases while DNA synthesized by the original chemistry (termed DMTS-std) had a large number of modifications. Under optimal reaction conditions, HhaI and RsaI cleaved the DMTS-std duplex to 76-77% completion and the DMTS-imp duplex to 96-99% completion. Restriction analysis and piperidine treatment yielded estimates of approximately 3.0% modified nucleotides in DMTS-std and approximately 1.0% in DMTS-imp. Overall, the improvements in chemistry increased the restriction efficiency of synthetic DNA up to 10-fold. | lld:pubmed |
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| pubmed-article:2537951 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2537951 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2537951 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2537951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2537951 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2537951 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2537951 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:2537951 | pubmed:author | pubmed-author:FarranceI KIK | lld:pubmed |
| pubmed-article:2537951 | pubmed:author | pubmed-author:IvarieRR | lld:pubmed |
| pubmed-article:2537951 | pubmed:author | pubmed-author:EadieJ SJS | lld:pubmed |
| pubmed-article:2537951 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2537951 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:2537951 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:2537951 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2537951 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2537951 | pubmed:pagination | 1231-45 | lld:pubmed |
| pubmed-article:2537951 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2537951 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2537951 | pubmed:articleTitle | Improved chemistry for oligodeoxyribonucleotide synthesis substantially improves restriction enzyme cleavage of a synthetic 35mer. | lld:pubmed |
| pubmed-article:2537951 | pubmed:affiliation | Department of Genetics, University of Georgia, Athens 30602. | lld:pubmed |
| pubmed-article:2537951 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2537951 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2537951 | lld:pubmed |
