|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1989-4-12
|
|
pubmed:abstractText |
Two DNA duplexes of identical sequence and 35 nt in length were synthesized by an original and a highly improved version of phosphoramidite chemistry. By base composition analysis, DNA synthesized by improved chemistry (termed DMTS-imp) contained no detectable modified bases while DNA synthesized by the original chemistry (termed DMTS-std) had a large number of modifications. Under optimal reaction conditions, HhaI and RsaI cleaved the DMTS-std duplex to 76-77% completion and the DMTS-imp duplex to 96-99% completion. Restriction analysis and piperidine treatment yielded estimates of approximately 3.0% modified nucleotides in DMTS-std and approximately 1.0% in DMTS-imp. Overall, the improvements in chemistry increased the restriction efficiency of synthetic DNA up to 10-fold.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-2827538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-2831625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-2948952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-3023872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-3039984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-3093895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-3126097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-3671086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-3748823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-4011433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-6300767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2537951-6357277
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Modification Methylases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Organophosphorus Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Piperidines,
http://linkedlifedata.com/resource/pubmed/chemical/Thymine,
http://linkedlifedata.com/resource/pubmed/chemical/diisopropyl phosphoramidite,
http://linkedlifedata.com/resource/pubmed/chemical/piperidine
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0305-1048
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
11
|
|
pubmed:volume |
17
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1231-45
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2537951-Adenine,
pubmed-meshheading:2537951-Base Composition,
pubmed-meshheading:2537951-Cloning, Molecular,
pubmed-meshheading:2537951-DNA,
pubmed-meshheading:2537951-DNA Modification Methylases,
pubmed-meshheading:2537951-DNA Restriction Enzymes,
pubmed-meshheading:2537951-Guanine,
pubmed-meshheading:2537951-Oligodeoxyribonucleotides,
pubmed-meshheading:2537951-Organophosphorus Compounds,
pubmed-meshheading:2537951-Piperidines,
pubmed-meshheading:2537951-Restriction Mapping,
pubmed-meshheading:2537951-Substrate Specificity,
pubmed-meshheading:2537951-Thymine
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Improved chemistry for oligodeoxyribonucleotide synthesis substantially improves restriction enzyme cleavage of a synthetic 35mer.
|
|
pubmed:affiliation |
Department of Genetics, University of Georgia, Athens 30602.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
