Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-3-16
pubmed:abstractText
Primary cell cultures from fetal bovine sternum were developed in Coon's modified Ham's F-12 medium containing 10% Nu-Serum, 1% Ultroser-G, and 200 mg of galactose per liter. Clones were obtained by colony isolation; one clone, BBE-1, was selected for characterization. BBE-1 cells exhibited typical endothelial morphology by light and electron microscopy and immunofluorescence for factor VIII-related antigen throughout their life span of 8 months. The cells showed mitogenic responses to endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor types I and II, platelet-derived growth factor, ascorbic acid, and progesterone. Parathyroid hormone stimulated intracellular accumulation of cAMP in BBE-1 cells but not in endothelial cells from two other tissues. These clonal cells provide a useful system for studies on bone vasculature, including its interactions with other bone cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-1113513, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-204011, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-2412230, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-2435393, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-2440047, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-2441387, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-2820420, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-2820761, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-2854747, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-2990870, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3461784, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3491656, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3541986, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3654763, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3690622, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3720664, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3759796, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3801237, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3855255, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3886167, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3927288, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3958059, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-3986914, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6120850, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6269927, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6304160, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6341464, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6389097, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6402043, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6441422, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6621711, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6827919, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-6994839, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-709401, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-7355268, http://linkedlifedata.com/resource/pubmed/commentcorrection/2536936-779999
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
916-20
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Cloned endothelial cells from fetal bovine bone.
pubmed:affiliation
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
pubmed:publicationType
Journal Article