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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-2-1
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated a chromosomal DNA segment of the human IL-4 gene based on homology with a human IL-4 cDNA sequence and determined its complete nucleotide sequence. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. It is composed of four exons and three introns and is approximately 10 kilobase pairs in size. 5'-Flanking regions of human and mouse IL-4 genes share about 85% homology extending more than 500 base pairs upstream of a "TATA" like sequence. Several patches of sequences are found in the 5'-flanking region of the human IL-4 gene which are homologous to sequence in the 5'-flanking regions of the IL-2, IL-3, IL-5, and granulocyte-macrophage (GM)-CSF genes. The IL-4 gene is inducible after treatment of human T cell clone by phorbol-12-myristate-13-acetate (TPA) and calcium ionophore A23187. The 2.3-kb 5'-flanking region of the human IL-4 gene transiently transfected into Jurkat human T cell leukemia cells is activated efficiently in response to TPA and A23187 stimulation and, although less efficiently, by human T cell leukemia virus type I-encoded p40x or BPV-encoded E2 protein. Combination of TPA/A23187 and p40x or E2 protein further augmented the level of expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E2 protein, Bovine papillomavirus,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
142
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
274-82
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2535858-Amino Acid Sequence,
pubmed-meshheading:2535858-Base Sequence,
pubmed-meshheading:2535858-Bovine papillomavirus 1,
pubmed-meshheading:2535858-Calcimycin,
pubmed-meshheading:2535858-Chromosomes, Human, Pair 5,
pubmed-meshheading:2535858-Clone Cells,
pubmed-meshheading:2535858-Cloning, Molecular,
pubmed-meshheading:2535858-DNA-Binding Proteins,
pubmed-meshheading:2535858-Gene Expression Regulation,
pubmed-meshheading:2535858-Genes,
pubmed-meshheading:2535858-Human T-lymphotropic virus 1,
pubmed-meshheading:2535858-Humans,
pubmed-meshheading:2535858-Interleukin-4,
pubmed-meshheading:2535858-Interleukins,
pubmed-meshheading:2535858-Lymphocyte Activation,
pubmed-meshheading:2535858-Molecular Sequence Data,
pubmed-meshheading:2535858-RNA, Messenger,
pubmed-meshheading:2535858-Retroviridae Proteins,
pubmed-meshheading:2535858-T-Lymphocytes,
pubmed-meshheading:2535858-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2535858-Transfection,
pubmed-meshheading:2535858-Viral Proteins
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pubmed:year |
1989
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pubmed:articleTitle |
Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression.
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pubmed:affiliation |
Department of Molecular Biology, DNAX Research Institute, Palo Alto, CA 94304-1104.
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pubmed:publicationType |
Journal Article
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