Linked Life Data

2535847

Source:http://linkedlifedata.com/resource/pubmed/id/2535847

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-2-7
pubmed:abstractText
Blot analysis of poly(A+) RNA showed a rapid and dramatic insulin induction of fatty acid synthase (FAS) mRNA in diabetic mouse liver. Insulin administration to diabetic mice increased the mRNA level 4-fold within 1 h, and a maximum of 19-fold was reached in 6 h. The nutritional induction of FAS mRNA by fasting/refeeding was abolished by streptozotocin-induced diabetes and also by dibutyryl cAMP administered during refeeding in normal animals, demonstrating the roles of insulin and cAMP during nutritional manipulation. Run-on transcription analysis with isolated nuclei showed that the transcription rate of the FAS gene increased 3.5-fold after 30 min, reached a maximum of 7-fold after 2 h of insulin administration in diabetic animals, and was maintained at the maximum level to 6 h. The cAMP, administered to previously fasted normal mice during refeeding of a high carbohydrate diet, abolished the increased transcription of the FAS gene caused by nutritional manipulation. These results indicate positive control by insulin and negative control by dibutyryl cAMP of FAS gene transcription. Furthermore, the effect of insulin on the FAS mRNA level was abolished by cycloheximide administration. This indicates that ongoing protein synthesis is necessary for the transcriptional activation of the FAS gene by insulin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
574-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Hormonal regulation of mouse fatty acid synthase gene transcription in liver.
pubmed:affiliation
Department of Nutrition, Harvard School of Public Health, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.