Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1989-10-17
pubmed:abstractText
When a bacterial plasmid containing the entire genome of LuIII virus except for the terminal 18 nucleotides from the right end is transfected into HeLa cells, the viral DNA is rescued and replicated, with production of infectious virus. This experimental system was used to examine the viral proteins and cis elements required for the excision and replication of viral DNA. The deletion of the entire NS1 gene provided a viral genome that was excised from the plasmid and replicated only when an NS1 gene was provided in trans. A frameshift mutation in the NS2 intron that truncates NS1 prevented excision and replication. Deletion of the left-end terminal inverted repeat or the right-end inverted repeat prevented excision of viral DNA from that end but not from the wild-type terminus. The viral terminus excised from the plasmid was protected from a processive degradation process, which began on the vector portion of the plasmid. The inhibitor of DNA polymerases alpha and delta, aphidicolin, blocked the excision reaction.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-13911591, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-232178, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-2537072, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-2542625, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-2826664, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-2841582, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-2845646, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-2850017, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-2946025, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-2969054, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3018288, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3019672, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3033274, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3037312, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3041032, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3203742, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3275788, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3296697, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3339715, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3491293, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3612951, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-3819702, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-4020972, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6086948, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6088052, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6088786, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6094825, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6098692, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6284985, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6345805, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6504049, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-6823009, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-691114, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-7097859, http://linkedlifedata.com/resource/pubmed/commentcorrection/2528644-851476
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4249-56
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Both excision and replication of cloned autonomous parvovirus DNA require the NS1 (rep) protein.
pubmed:affiliation
Eppley Institute, University of Nebraska Medical Center, Omaha 68105-1065.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't